rabbit anti pfkfb3  (Proteintech)

Journal: Pharmaceuticals

Article Title: Investigating the Role of Glycolysis in Xuefu Zhuyu Capsule-Promoted Angiogenesis in Endothelial Cells: A Study Based on Network Pharmacology, Molecular Docking, and In Vitro Validation

doi: 10.3390/ph18121902

Figure Lengend Snippet: Molecular Docking Visualization of Key Active Components from XFZYC with PFKFB3. ( A ) Kaempferol (MOL000422), ( B ) Jaranol (MOL000239), ( C ) Lupiwighteone (MOL003656), ( D ) 7-Methoxy-2-methyl isoflavone (MOL003896), ( E ) Euchrenone (MOL004806), ( F ) Gancaonin A (MOL004856), ( G ) Shinpterocarpin (MOL004891), ( H ) 7-Acetoxy-2-methylisoflavone (MOL004991). In the figure, steel blue denotes the PFKFB3 protein, yellow represents the small-molecule compound, and green indicates the specific docking site.

Article Snippet: Rabbit anti-β-actin and rabbit anti-PFKFB3 antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA (Product Nos.

Techniques:

Journal: Pharmaceuticals

Article Title: Investigating the Role of Glycolysis in Xuefu Zhuyu Capsule-Promoted Angiogenesis in Endothelial Cells: A Study Based on Network Pharmacology, Molecular Docking, and In Vitro Validation

doi: 10.3390/ph18121902

Figure Lengend Snippet: Effects of different concentrations of Xuefu Zhuyu Capsule drug-containing serum on endothelial cell tube formation capacity and HIF-1α, HK2, and PFKFB3 protein expression. ( A , C ) Tube formation assay detecting the in vitro tube formation capacity of endothelial cells treated with different concentrations of XFZYC drug-containing serum. ( B , D , E , F ) Protein expression levels of PFKFB3, HK2, and HIF-1α under treatment with 1.25%, 2.5%, and 5% blank serum or drug-containing serum were determined by Western blot analysis. * p < 0.05 for the 1.25% XFZYC group compared to the 1.25% Blank group; # p < 0.05, #### p < 0.0001 for the 2.5% XFZYC group compared to the 2.5% Blank group, ns, not significant; n = 3.

Article Snippet: Rabbit anti-β-actin and rabbit anti-PFKFB3 antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA (Product Nos.

Techniques: Expressing, Tube Formation Assay, In Vitro, Western Blot

Journal: Pharmaceuticals

Article Title: Investigating the Role of Glycolysis in Xuefu Zhuyu Capsule-Promoted Angiogenesis in Endothelial Cells: A Study Based on Network Pharmacology, Molecular Docking, and In Vitro Validation

doi: 10.3390/ph18121902

Figure Lengend Snippet: Effects of XFZYC-containing serum and the glycolytic inhibitor 3PO on glycolysis in endothelial cells. ( A ) Cell viability was determined by CCK-8 assay. ( B ) Glucose uptake was measured using a Glucose Assay Kit. ( C ) Lactate production was detected using a Lactate Assay Kit. ( D ) Citrate level was assessed using a Citrate Assay Kit. ( E ) HK enzyme activity was measured with a Hexokinase Activity Assay Kit. ( F ) PFK enzyme activity was determined using a Phosphofructokinase Activity Assay Kit. ( G ) PK enzyme activity was assessed with a Pyruvate Kinase Activity Assay Kit. ( H , I ) PFKFB3 protein expression was determined by Western blot analysis. * p < 0.05, ** p < 0.01, **** p < 0.0001vs. Blank group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.001 vs. XFZYC group; &&& p < 0.001 vs. XFZYC + 3PO group; ns, not significant; n = 3.

Article Snippet: Rabbit anti-β-actin and rabbit anti-PFKFB3 antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA (Product Nos.

Techniques: CCK-8 Assay, Glucose Assay, Lactate Assay, Citrate Assay, Activity Assay, Kinase Assay, Expressing, Western Blot